|
KMID : 0903519700130030231
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1970 Volume.13 No. 3 p.231 ~ p.235
|
|
|
Studies on Enzyme of the Thermophilic Mold
|
|
Lee Ke-Ho
|
|
|
Abstract
|
|
|
1. Purification of amylase system produced from Humicola sp. by a submerged culture eras carried out.
2. By DEAE-Cellulose column chromatography amylase system was separated into two fractions eluted at 0.05M and 0.5M phosphate buffer solution of pH 6.0.
3. The saccharogenic amylase was mostly composed of. the fraction of 0.05M phosphate buffer solution of pH 6.0 while the dextrinogenic amylase was perseted in fraction of 0.5M phosphate buffer solution of pH 6.0
4. It was found that the optimum pH of this saccharogenic amylase was within the range of from 4.5 to 5.5, stable pH was within the range of from 4.0 to 9.0 and optimum temperature was 60-65℃. This amylase was stable at 70℃ for ten minutes but completely inactivated 80℃ above.
Acknowlegdement; The author wishes to express his thanks to Dr. Mun, Hui Sok, President of Kon-Kuk University, for research fund and professor Kang, Hyo Won in our laboratory for his valuable suggestion and discussion.
This study was supported a part by a Grant in Aid for Scientific Research from Kon-Kuk University.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|